熒光原位雜交（FISH）是一種利用熒光標記的核酸片段為探針，與組織、細胞或染色體上待測DNA或RNA互補配對，結合成專一的核酸雜交分子，通過熒光檢測系統將待測核酸在組織、細胞或染色體中的位置顯示出來的技術。
由銳博生物自主研發的RNA FISH檢測探針（FISH Probe Mix），創新性地改進了RNA分子的特異性核酸探針數量及標記方式，極大地提高了探針靈敏度，能滿足細胞中RNA的準確定位要求，結合激光共聚焦顯微鏡可清晰地展示RNA的分布情況，為RNA功能及作用機制研究提供強有力的工具。此外，還可搭配FISH必備的RiboTM?FISH試劑盒和FISH內參對照（18S或U6），真正讓您的實驗事半功倍。
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由于FISH Probe Mix具有如下特點：
◎?定位準確，核內核外清晰明了，功能機制更易確定
◎?靈敏度高，信號放大倍數平均達到160倍，輕松定位低拷貝數RNA
◎?特異性強，集群式的FISH探針能夠最大限度地保證FISH信號的高特異性
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因此，自FISH產品上市以來，得到了國內外廣大客戶的認可與信賴，使用銳博FISH產品發表的SCI文章已有2000多篇，越來越多的客戶文章發表在了諸如Nature Cell Biology、Nature Metabolism、Cell Stem Cell、Cell Research、Molecular Cell、Science Advances、Nature Communications、Signal Transduction and Targeted Therapy、Advanced Science等國際知名期刊。
而眾所周知，銳博FISH產品是可以用于細胞定位的，已經有很多客戶發表的高分文章成功案例（見文末：FISH細胞定位成功案例客戶高分文獻）。最近，小編收到了很多客戶咨詢該FISH產品是否可以用于組織檢測？以及是否有成功的案例可以參考？今天就給大家簡單整理了幾篇銳博FISH探針用于組織定位檢測的客戶文章，希望可以對各位小伙伴有所幫助，也相信使用這個RNA FISH定位分析方法，可以給您的研究帶來更多不一樣的驚喜！
客戶應用案例1：
Cell Research丨A novel pathway regulates social hierarchy via lncRNA AtLAS and postsynaptic synapsin IIb
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研究模型：社會等級調控
目標分子：lncRNA AtLAS
組織類型：小鼠腦切片
實驗目的：為了探索lncRNA是否以及如何參與社會等級的建立，通過特定的RNA探針進行FISH檢測以了解AtLAS的組織和細胞定位
實驗結果：AtLAS位于細胞核和細胞質區域，并分布在整個大腦中
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(e) Representative images of fluorescent in situ hybridizations (FISH) of AtLAS co-labeled with DAPI (blue) in cultured prefrontal cortical (PFC) neurons. 18S rRNA as the cytoplasmic control.
(f) Representative images of FISH of AtLAS in medial prefrontal cortex (mPFC) co-labeled with DAPI (blue).
(g) AtLAS expression profiles in different brain areas detected by qRT-PCR. Ob, olfactory bulb; Str, striatum; Hp, hippocampus.
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參考文獻：Ma M, Xiong W, Hu F, et al. A novel pathway regulates social hierarchy via lncRNA AtLAS and postsynaptic synapsin IIb[J].?Cell research, 2020, 30(2): 105-118.
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客戶應用案例2：
Cell Stem Cell丨A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis
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研究模型：心肌分化、心臟發生
目標分子：Linc1405
組織類型：小鼠E10.5胚胎心臟區域
實驗目的：為了探索linc1405對心肌分化的重要作用，通過FISH實驗驗證linc1405在心臟中的表達情況
實驗結果：linc1405顯示在E10.5胚胎心臟區域表達，暗示linc1405可能對心臟發育至關重要
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(A) Histograms showing the relative expression levels of linc1405 in fetal tissues (heart, hindleg, foreleg, brain, kidney, stomach, liver, and lung) from E16.5 mouse embryos.
(B) Representative image of FISH for linc1405 (red) in the heart area of E10.5 embryos.
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參考文獻：Guo X, Xu Y, Wang Z, et al. A Linc1405/Eomes complex promotes cardiac mesoderm specification and cardiogenesis[J].?Cell Stem Cell, 2018, 22(6): 893-908. e6.
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客戶應用案例3：
Molecular Therapy丨FOXO3A-induced LINC00926 suppresses breast tumor growth and metastasis through inhibition of PGK1-mediated Warburg effect
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研究模型：乳腺癌生長和轉移
目標分子：LINC00926
組織類型：BC組織（石蠟組織切片）
實驗目的：為了探索乳腺癌中調控PGK1的上游lncRNAs，通過FISH檢測109個人類乳腺癌樣本中LINC00926的表達
實驗結果：LINC00926的表達與PGK1的表達呈負相關，與FOXO3A的表達呈正相關。暗示LINC00926/PGK1軸在乳腺癌中的重要病理作用
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(A) Representative IHC staining for FOXO3A and PGK1 and FISH staining for LINC00926 in BC patients. CASE 1 and CASE 2 refer to two representative samples categorized by high and low LINC00926 expression.
(B) The correlation of glucose uptake in breast cancer patients with FOXO3A and LINC00926 was determined using the Mann Whitney U test. CASE 1 and CASE 2 refer to two representative samples categorized by high and low FDG uptake.?
參考文獻：Chu Z, Huo N, Zhu X, et al. FOXO3A-induced LINC00926 suppresses breast tumor growth and metastasis through inhibition of PGK1-mediated Warburg effect[J].?Molecular Therapy, 2021.
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客戶應用案例4：
Theranostics丨CircCDK14 protects against Osteoarthritis by sponging miR-125a-5p and promoting the expression of Smad2
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研究模型：骨關節炎（OA）
目標分子：CircCDK14
組織類型：軟骨樣本
實驗目的：為了探索新型circRNA因子CircCDK14在OA發病過程中的作用，并闡明其分子機制。通過RNA FISH檢測人類樣本中負重區和非負重區CircCDK14的表達水平
實驗結果：與非負重區相比，CircCDK14 在關節負重區的表達顯著下調
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(C) Expression levels of CircCDK14 was detected by RNA FISH in weight bearing area and non-weight bearing area in human samples.
參考文獻：Shen P, Yang Y, Liu G, et al. CircCDK14 protects against Osteoarthritis by sponging miR-125a-5p and promoting the expression of Smad2[J].?Theranostics, 2020, 10(20): 9113.
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其他用于組織檢測的成功案例還有：
[1] Xu Q, Guohui M, Li D, et al. lncRNA C2dat2 facilitates autophagy and apoptosis via the miR-30d-5p/DDIT4/mTOR axis in cerebral ischemia-reperfusion injury[J].?Aging (Albany NY), 2021, 13(8): 11315.
[2] Chen Z, Zhuang Q, Cheng K, et al. Long non-coding RNA TCL6 enhances preferential toxicity of paclitaxel to renal cell carcinoma cells[J].?Journal of Cancer, 2020, 11(6): 1383.
[3] Huang W, Shi Y, Han B, et al. LncRNA GAS5-AS1 inhibits glioma proliferation, migration, and invasion via miR‐106b-5p/TUSC2 axis[J].?Human cell, 2020, 33(2): 416-426.
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FISH細胞定位成功案例客戶高分文獻：
[1] Chen S, Zhu G, Yang Y, et al. Single-cell analysis reveals transcriptomic remodellings in distinct cell types that contribute to human prostate cancer progression[J].?Nature Cell Biology, 2021, 23(1): 87-98.
[2] Sang L, Ju H, Yang Z, et al. Mitochondrial long non-coding RNA GAS5 tunes TCA metabolism in response to nutrient stress[J].?Nature Metabolism, 2021, 3(1): 90-106.
[3] Shi L, Liu B, Shen D, et al. A tumor-suppressive circular RNA mediates uncanonical integrin degradation by the proteasome in liver cancer[J].?Science Advances, 2021, 7(13): eabe5043.
[4] Li Y, Chen B, Zhao J, et al. HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein[J].?Advanced Science, 2021: 2001701.
[5] Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J].?Developmental Cell,?2021, 56(5): 702-715. e8.
[6] Xie F, Huang C, Liu F, et al. CircPTPRA blocks the recognition of RNA N 6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression[J].?Molecular cancer, 2021, 20(1): 1-17.
[7] Shen P, Yang T, Chen Q, et al. CircNEIL3 regulatory loop promotes pancreatic ductal adenocarcinoma progression via miRNA sponging and A-to-I RNA-editing[J].?Molecular cancer, 2021, 20(1): 1-22.
[8] Cen J, Liang Y, Huang Y, et al. Circular RNA circSDHC serves as a sponge for miR-127-3p to promote the proliferation and metastasis of renal cell carcinoma via the CDKN3/E2F1 axis[J].?Molecular cancer, 2021, 20(1): 1-14.
[9] Xu Y, Xi J, Wang G, et al. PAUPAR and PAX6 sequentially regulate human embryonic stem cell cortical differentiation[J].?Nucleic acids research, 2021, 49(4): 1935-1950.
[10] Liu J, Liu Z X, Wu Q N, et al. Long noncoding RNA AGPG regulates PFKFB3-mediated tumor glycolytic reprogramming[J].?Nature communications, 2020, 11(1): 1-16.
[11] Dong Z, Gao M, Li C, et al. LncRNA UCA1 Antagonizes Arsenic‐Induced Cell Cycle Arrest through Destabilizing EZH2 and Facilitating NFATc2 Expression[J].?Advanced Science, 2020, 7(11): 1903630.
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